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UNIV - Research Specialist III - Pediatrics: Children's Research Institute

Apply now Job no: 520460
Entity: MUSC University
Department: COLLEGE OF MEDICINE - COM-Pediatrics
Sub Department: UNIV-2248960-Childrens Research Institute
Work type: Full-Time
Employee Type: UNIV - Research Grant
Location: Charleston, SC
Categories: RESEARCH
Pay scale: UNIV-Band 6: $40,759.00 - $58,086.00 - $75,413.00 (min - mid - max)

Job Duties:
10Participate in laboratory and scientific meetings, and manuscript and grant preparation.Essential
10Responsible for keeping accurate and timely records of all activities, including experimental data, image analysis.Essential
20Maintain several cell cultures and primary cultures in vitro. Bacterial recombinant DNA work and lentiviral production for in vitro transfection and transduction experiments. Perform a number of cell and molecular biology procedures, such as cell viability, IF, FACs analyses, immunoblotting, PCR, qPCR, reporter assay.Essential
20Mouse husbandry, colony management and genotyping including sample collection and preparation, and polymerase chain reaction (PCR) genotyping analysis. Complete small animal surgeries and live imaging analyses. Complete xenograft and allograft mouse tumor models, including implantation of tumor cells in the brain, compound dosing, and in vivo tumor growth assessment. Tissue and cell isolation from mice, followed by immunohistochemistry, microscopy and a variety of cellular and molecular biology procedures.Essential
30Manage multiple projects with minimal guidance from PI. Responsibilities include designing, setting-up and running experiments in consultation with the Principal Investigator and effectively recording, analyzing, acquiring and analysis. The PI provides an overview of the hypothesis and overall plans. The Research Specialist then plans detailed experiments and completes them. The candidate will be expected to troubleshoot experiments.Essential
5Collaborate on ordering of laboratory supplies, maintaining stocks of experimental reagents and solutions, maintaining equipment in good repair, glassware washing and general lab cleanliness.Essential
5Oversee undergraduate, graduate students and postdoctoral fellows in lab.Essential


Job summary: Cancer research laboratory in the Darby Children’s Research Institute at the Department of Pediatrics has a job opening for a research technician position. The lab uses in vitro, ex vivo and in vivo models to elucidate the intracellular drivers of pediatric brain tumor growth.

The candidate will be responsible for managing the lab, lab staff and multiple projects with minimal guidance from the PI, and in addition to running the lab in the absence of the PI. They will be responsible for designing, setting up, and processing experiments using an array of molecular and cellular biology techniques (including RT-qPCR, ELISA, immunoprecipitations (including ChiP), western blotting, cloning / transfections / lentivirus infections). They will be expected to have experience in cell cultures; specifically, they will be expected to maintain cell lines and also grow brain primary cultures. They should have broad knowledge of immunohistochemistry, immunofluorescence staining in both tissue and cell culture, confocal microscopy and FACS analysis.

Candidate should have extensive experience handling mice, including experience on transgenic mouse colony maintenance, in vivo survival and non-survival surgeries and drug administration (intraperitoneal, intravenous, subcutaneous and oral gavage). In addition, they will be expected to help on manuscript preparation and submission to refereed journals and to present at scientific meetings.

Some knowledge in cancer intracellular signaling, developmental signaling including SHH signaling, brain tumor ex vivo and in vivo modeling and immunohistochemistry is expected.

Candidate should send CV and a list of three references directly to Dr. Jezabel R Blanco by email (rblanco@musc.edu).


Payscale Salary Range: UNIV-Band 6: $40,759.00 - $58,086.00 - $75,413.00 (min - mid - max)


FLSA: Salaried


Minimum Experience and Training Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science directly related to the assigned area of research and two years advanced journel-level work experience in a clinical or research setting.


Physical Requirements: Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32? F to 100? F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/20 vision, corrected. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous)

The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.


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Applications close: Eastern Daylight Time

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The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need.