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Molecular Biology and Protein Chemistry
Perform recombinant DNA techniques including isolation and purification of plasmid DNA and genomic DNA, restriction enzymatic analysis of DNA, PCR, qPCR, bacterial transformations, agarose gel electrophoresis, and transformation; Protein Biochemistry including SDS-PAGE, Western Blotting and ELISA. Prepare biological samples and conduct mass photometry analysis.
Cell Culture and Cellular Biology
Perform research experiments investigating the mechanisms of muscle cell development; perform mammalian cell transfections; mammalian cell culture techniques; confocal microscopy; high-throughput cell assays.
Research Project Coordination
Serves a liaison between PI/Director, lab staff, and collaborators within as well as external to UF. Coordinates special studies or projects as directed; responsible for coordinating activities of all ongoing research projects. Responsible for the complete understanding and compliance with all required laboratory regulations such as ACS, EH&S, IACUC, UF, State and Federal guidelines Maintains accurate records and legal documentation required by institutional policies such as biological, chemical, and radiation safety policies. Manage ordering process to ensure efficient and cost-effective use of grant funding; maintain lab supply inventory to ensure supplies are available for scheduled research projects; researches and evaluates new products, compares and negotiates vendor prices.
Scientific Writing and Dissemination
Analyze data and assemble results for scientific publications, grants, and conference presentations; assist in preparing data for scientific manuscripts, abstracts, grants, and other documents; manage accurate and complete laboratory research records and prepares daily written reports of experimental results; assists laboratory research with searches for and review of scientific literature relevant to the research focus of the laboratory. Aids in the development of new research and laboratory protocols
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