Core Rsch Facil Technician III - NextGen DNA Sequencing
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Job no: 539525
Work type: Staff Full-Time
Location: Main Campus (Gainesville, FL)
Categories: Biology/Life Science, Grant or Research Administration, Physical/Mathematical Sciences
Department:11600107 - RE-ICBR-NEXTGEN
| Classification Title: |
Core Rsch Facil Technician III
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| Job Description: |
Technical Functions
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Performs QC of samples submitted to the NGS lab. This includes assessing the quantity and quality of materials to determine suitability for NextGen DNA sequencing procedures.
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Submits samples for qPCR to the Gene Expression core
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Constructs Illumina libraries for sequencing on Illumina platforms
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Properly tracks samples and documents laboratory work
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Communicates with supervisor and other core personnel about experimental results in a timely and professional manner
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Works with the supervisor to define deliverables and timelines for project completion
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Tests, troubleshoots, and implements new protocols for library construction, DNA shearing, sample cleanup, and size selection, using novel procedures as they become available in the literature or from vendors
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Contributes to the preparation, updating, and organization of Standard Operating Procedures (SOPs)
General Lab Operation and Maintenance Functions
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Performs maintenance tasks associated with the upkeep of the NGS lab, such as the proper disposal of biohazard trash, receiving and storing of samples and reagents
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Monitors reagents and consumables and regularly updates inventories
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Assists with the administrative duties necessary for proper handling of receipts and orders
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Revision of sample sheets, tracking, and disposal of samples in MyBiotech
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Freeze and fridge maintenance and documentation in MyBiotech
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Other duties as assigned
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| Expected Salary: |
$45,000 - $46,000
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| Required Qualifications: |
BA/BS in a related scientific field, or an equivalent combination of education and experience. Core or technology-specific licensure and/or certifications, where applicable. Additionally, yearly certificate and compliance training, if required.
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| Preferred: |
The preferred applicant will have a BS/BA in Molecular Biology or a related scientific field with familiarity of in basic techniques and instrumentation used in the molecular biology laboratory, including specific NGS procedures that include (but are not limited to):
- Genomic DNA purification, quantification, and manipulation; PCR; NGS DNA library construction and quality assessment/evaluation.
- Use of robotics for high-throughput processing of nucleic acids and DNA library samples.
- Hands-on experience with Illumina, PacBio, and/or ONT NGS instrumentation and technology.
- Basic laboratory citizenship, including organization of equipment and supply inventory.
- Instrument run coordination and maintenance.
- Preparation of common buffers and solutions.
- Computer literacy in Windows/UNIX systems, Microsoft Office package.
- Familiarity with utilizing Standard Operating Procedures (SOPs), iLAB, and laboratory information management system (LIMS).
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| Special Instructions to Applicants: |
In order to be considered, you must upload your cover letter and resume.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.
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| Health Assessment Required: |
No |
Advertised: Eastern Daylight Time
Applications close: Eastern Daylight Time
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