Core Rsch Facil Technician III - NextGen DNA Sequencing

Apply now Job no: 539525
Work type: Staff Full-Time
Location: Main Campus (Gainesville, FL)
Categories: Biology/Life Science, Grant or Research Administration, Physical/Mathematical Sciences
Department:11600107 - RE-ICBR-NEXTGEN

Classification Title:

Core Rsch Facil Technician III

Job Description:

Technical Functions

  • Performs QC of samples submitted to the NGS lab. This includes assessing the quantity and quality of materials to determine suitability for NextGen DNA sequencing procedures.

  • Submits samples for qPCR to the Gene Expression core

  • Constructs Illumina libraries for sequencing on Illumina platforms

  • Properly tracks samples and documents laboratory work

  • Communicates with supervisor and other core personnel about experimental results in a timely and professional manner

  • Works with the supervisor to define deliverables and timelines for project completion

  • Tests, troubleshoots, and implements new protocols for library construction, DNA shearing, sample cleanup, and size selection, using novel procedures as they become available in the literature or from vendors

  • Contributes to the preparation, updating, and organization of Standard Operating Procedures (SOPs)

General Lab Operation and Maintenance Functions

  • Performs maintenance tasks associated with the upkeep of the NGS lab, such as the proper disposal of biohazard trash, receiving and storing of samples and reagents

  • Monitors reagents and consumables and regularly updates inventories

  • Assists with the administrative duties necessary for proper handling of receipts and orders

  • Revision of sample sheets, tracking, and disposal of samples in MyBiotech

  • Freeze and fridge maintenance and documentation in MyBiotech

  • Other duties as assigned

Expected Salary:

$45,000 - $46,000

Required Qualifications:

BA/BS in a related scientific field, or an equivalent combination of education and experience.
Core or technology-specific licensure and/or certifications, where applicable. Additionally, yearly certificate and compliance training, if required.

Preferred:

The preferred applicant will have a BS/BA in Molecular Biology or a related scientific field with  familiarity of in basic techniques and instrumentation used in the molecular biology laboratory, including specific NGS procedures that include (but are not limited to):

  • Genomic DNA purification, quantification, and manipulation; PCR; NGS DNA library construction and quality assessment/evaluation.
  • Use of robotics for high-throughput processing of nucleic acids and DNA library samples.
  • Hands-on experience with Illumina, PacBio, and/or ONT NGS instrumentation and technology.
  • Basic laboratory citizenship, including organization of equipment and supply inventory.
  • Instrument run coordination and maintenance.
  • Preparation of common buffers and solutions.
  • Computer literacy in Windows/UNIX systems, Microsoft Office package.
  • Familiarity with utilizing Standard Operating Procedures (SOPs), iLAB, and laboratory information management system (LIMS).
Special Instructions to Applicants:

In order to be considered, you must upload your cover letter and resume. 

Application must be submitted by 11:55 p.m. (ET) of the posting end date.

Health Assessment Required: No

 

Advertised: Eastern Daylight Time
Applications close: Eastern Daylight Time

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