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RNA scope and Protein Chemistry
Plan, design, and conduct advanced research projects primarily involving RNA-scope of neurons and wide
field microscopy. Perform recombinant DNA techniques including isolation and purification of plasmid DNA,
restriction enzymatic analysis of DNA, PCR, qPCR, bacterial transformations, agarose gel electrophoresis,
and transformation; Protein Biochemistry including SDS-PAGE, Western Blotting and ELISA.
Cell Culture and Cellular Biology
Perform research experiments investigating mechanisms of action of channel interfering compounds in
rodent nociceptors; perform mammalian cell transfections; mammalian cell culture techniques; confocal
and wide-field microscopy.
Research Project Coordination
Serves a liaison between PI/Director, lab staff, and collaborators within as well as external to UF.
Coordinates special studies or projects as directed; responsible for coordinating activities of all ongoing
research projects. Responsible for the complete understanding and compliance with all required laboratory
regulations such as ACS, EH&S, IACUC, UF, State and Federal guidelines Maintains accurate records and
legal documentation required by institutional policies such as biological, and chemical safety policies.
Manage ordering process to ensure efficient and cost-effective use of grant funding; maintain lab supply
inventory to ensure supplies are available for scheduled research projects; researches and evaluates new
products, compares and negotiates vendor prices.
Scientific Writing and Dissemination
Analyze data and assemble results for scientific publications, grants, and conference presentations; assist
in preparing data for scientific manuscripts, abstracts, grants, and other documents; manage accurate and
complete laboratory research records and prepares daily written reports of experimental results; assists
laboratory research with searches for and review of scientific literature relevant to the research focus of the
laboratory. Aids in the development of new research and laboratory protocols.
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