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RNA scope and Protein Chemistry
Plan, design, conduct, and modify advanced research projects primarily involving RNA-scope of neurons and wide-field microscopy, ensuring execution aligns with research goals. Search scientific literature to refine procedures in line with research objectives. Perform recombinant DNA techniques, including isolation and purification of plasmid DNA, restriction enzymatic analysis of DNA, PCR, qPCR, bacterial transformations, agarose gel electrophoresis, and transformation; Protein Biochemistry, including SDS-PAGE, Western Blotting and ELISA. Conduct all research in line with quality and safety standards.
Cell Culture and Cellular Biology
Design and perform research experiments investigating mechanisms of action of channel-interfering compounds in rodent nociceptors, including the study of live specimens under controlled laboratory conditions; perform mammalian cell transfections; mammalian cell culture techniques, confocal and wide-field microscopy. Modify computational and analytical techniques as needed to meet research objectives.
Research Project Coordination
Acts as a liaison between the PI/Director, lab staff, and collaborators within UF and externally. Coordinates activities for ongoing government- and privately funded research projects and assists with special studies or projects as assigned. Assists in laboratory coordination and equipment maintenance and supports the training of junior laboratory personnel and students. Ensures understanding of and compliance with required laboratory regulations, including ACS, EH&S, IACUC, UF, State, and Federal guidelines.
Maintains accurate records and legal documentation in line with institutional policies, such as biological and chemical safety policies. Supports the ordering process to promote efficient and cost-effective use of grant funding, maintains lab supply inventory for scheduled research projects, and assists with researching products and comparing vendor prices.
Scientific Writing and Dissemination
Analyze data using appropriate statistical models and adjust research designs based on findings; assemble results for scientific publications, grants, and conference presentations; contribute to research papers, reports, and scholarly articles; manage accurate and complete laboratory research records and prepare written reports of experimental results; conduct searches for and review of scientific literature relevant to the research focus of the laboratory. Develops and troubleshoots new research and laboratory protocols. Engages in continuous learning and adjusts to evolving research needs.
Other duties as assigned
Works with a degree of independence under the general supervision of the Principal Investigator, following standard concepts, practices, and procedures within the biological field; seeking guidance from the Principal Investigator for complex issues. This position may include supervisory responsibility: may supervise and train Biological Scientist I staff, OPS laboratory technicians, and student assistants as assigned (position numbers to be determined).
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