Perform tissue processing including receipt of tissue, gross and fine dissection, tissue fixation, freezing, and sample storage. FFPE microtomy and OCT frozen sectioning of nPOD and CaRe-T1D case samples, as well as for off-site investigator sample requests. Embed sectioned tissue of pancreas, heart, vasculature, spleen, kidney, lymph nodes, and duodenum. Monthly completion of preventative maintenance of embedding station and organization of embedding area. Provide on-call tissue grossing support for the nPOD and CARE-T1D programs.
Perform manual special staining techniques, such as Trichrome, Pentachrome, and Verhoeff van Gieson stains. Assist in the operation and maintenance of automatic H&E and Immunohistochemistry staining machines. QC quality of staining in scanned slide inventory database.
Respond to sample requests. Assist with sample archiving and retrieval for distribution. Operate multiple laboratory information management systems for sample tracking and histology. Assist in coordinating sample distributions and sample inventory management
Assist with routine lab maintenance including ordering supplies, calibrating and checking equipment for proper function. Assist with periodic preventative maintenance on Core instruments. Troubleshoot equipment failures and arrange for or help with repairs. Evaluate new equipment to determine if it meets OPPC specific actions.
Review scientific literature to maintain and further understanding of Core technologies.
Assist in training and assessing new OPPC staff. Communicate with internal and external clients regarding OPPC and nPOD. Edit and write SOPs.
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