• Job Type: Support Staff - Union
• Bargaining Unit: SSA
• Regular/Temporary: Regular
• End Date if Temporary:
• Hours Per Week: 35
• Standard Work Schedule: 9:30am - 5:30pm
• Building: ICRC
• Salary Range: 51,839.00 - 51,839.00

Position Summary

The Technician B position will assist with cell culture, molecular and preclinical animal studies. The position involves processing of primary tissue samples and culture/analysis of cells. The candidate should be motivated to participate in sample processing both from preclinical modeling experiments as well as therapeutic studies. This position affords the motivated technician an opportunity to develop/utilize both tissue culture and molecular biology skills. 

Responsibilities

• Husbandry and maintenance of animal colony
• Weekly palpation and ultrasound of study mice 
• Necropsy of sick and study mice followed by organoid isolation, 2D cell establishment, biobanking (FFPE, OCT, snap-frozen tissue, cryopreserved tumor pieces, plasma), and histology submission. Update necropsy records accordingly.
• Establish and cryopreserve lab common mouse and human organoid lines
• Prepare and QC Rspondin/Wnt/Noggin conditioned media for organoid culture
• Purchasing and maintenance of lab reagents and instruments
• Monitor and maintain backup supply of all common lab reagents, liquid nitrogen, and office supplies
• Maintain lab inventories of reagents
• Liaise with sales reps and service technicians on pricing and maintenance of lab instruments

Minimum Qualifications

Bachelor's degree is required in chemistry, biology, physics or related science or an equivalent combination of education, training and experience.

Preferred Qualifications

Experience with mouse models, in vivo drug administration, cell biology assays e.g. flow cytometry, and/or molecular biology techniques is desired. Previous experience in 3D cell culture.

Other Requirements

Candidates with excellent organizational skills and communication skills (written and oral) plus an ability to work well in a multidisciplinary team environment are encouraged to apply. To be successful in this position, the candidate must be highly motivated, possess a high level of initiative and attention to details. The candidate must also be capable of working independently in a team environment.

Equal Opportunity Employer / Disability / Veteran

Columbia University is committed to the hiring of qualified local residents.